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Image Search Results
Journal: Human Gene Therapy
Article Title: Chimeric Receptor mRNA Transfection as a Tool to Generate Antineoplastic Lymphocytes
doi: 10.1089/hum.2008.068
Figure Lengend Snippet: CIR mRNA activity under various conditions. CTLs were obtained after short (1 day) or standard (7 days) ex vivo activation with anti-CD3/CD28 beads and IL-2, and were electroporated with CIR mRNA or without mRNA (mock). Surface CIR expression was visualized with a goat anti-mouse (Fab)2 polyclonal antibody conjugated with biotin and streptavidin PerCP (x axes). (A) Expression of anti-CD19 CIR in CTLs activated for 1 day (panel 1) or for 7 days (panel 2) (Elion et al., 2007); expression of anti-CD19 CIR δ(4–1BB) in CTLs activated for 7 days (panel 3). (B) Cytotoxicity of CTLs under the conditions indicated in (A). Targets, CD19+ autologous B cells, were loaded with 51Cr and analyzed at the indicated E:T ratios.
Article Snippet: Surface CIR expression was visualized with a goat anti-mouse (Fab) 2 polyclonal antibody conjugated with biotin and
Techniques: Activity Assay, Ex Vivo, Activation Assay, Expressing